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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
06/01/2023 |
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Data da última atualização: |
06/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FRIHLING, B. E. F.; BOLETI, A. P. de A.; OLIVEIRA, C. F. R. de; SANCHES, S. C.; CARDOSO, P. H. de O.; VERBISCK, N. V.; MACEDO, M. L. R.; SANTA RITA, P. H.; CARVALHO, C. M. E.; MIGLIOLO, L. |
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Afiliação: |
BRENO EMANUEL FARIAS FRIHLING, UNIVERSIDADE CATÓLICA DOM BOSCO; ANA PAULA DE ARAÚJO BOLETI, UNIVERSIDADE CATÓLICA DOM BOSCO; CAIO FERNANDO RAMALHO DE OLIVEIRA, UNIVERSIDADE FEDERAL DE MATO GROSSO DO SUL; SIMONE CAMARGO SANCHES, UNIVERSIDADE CATÓLICA DOM BOSCO; PEDRO HENRIQUE DE OLIVEIRA CARDOSO, UNIVERSIDADE CATÓLICA DOM BOSCO; NEWTON VALERIO VERBISCK, CNPGC; MARIA LÍGIA RODRIGUES MACEDO, UNIVERSIDADE FEDERAL DE MATO GROSSO DO SUL; PAULA HELENA SANTA RITA, UNIVERSIDADE CATÓLICA DOM BOSCO; CRISTIANO MARCELO ESPINOLA CARVALHO, UNIVERSIDADE CATÓLICA DOM BOSCO; LUDOVICO MIGLIOLO, UNIVERSIDADE FEDERAL DO RIO GRANDE DO NORTE. |
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Título: |
Purification, characterization and evaluation of the antitumoral activity of a phospholipase A2 from the snake Bothrops moojeni. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Pharmaceuticals, v. 15, n. 6, article 724, 2022. |
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DOI: |
https://doi.org/10.3390/ph15060724 |
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Idioma: |
Inglês |
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Notas: |
Na publicação: Newton Verbisck. |
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Conteúdo: |
Nature presents a wide range of biomolecules with pharmacological potential, including venomous animal proteins. Among the protein components from snake venoms, phospholipases (PLA2) are of great importance for the development of new anticancer compounds. Thus, we aimed to evaluate the PLA2 anticancer properties from Bothrops moojeni venom. The crude venom was purified through three chromatographic steps, monitored by enzymatic activity and SDS-PAGE (12%). The purified PLA2 denominated BmPLA2 had its molecular mass and N-terminal sequence identified by mass spectrometry and Edman degradation, respectively. BmPLA2 was assayed against human epithelial colorectal adenocarcinoma cells (Caco-2), human rhabdomyosarcoma cells (RD) and mucoepidermoid carcinoma of the lung (NCI-H292), using human fibroblast cells (MRC-5) and microglia cells (BV-2) as a cytotoxicity control. BmPLA2 presented 13,836 Da and a 24 amino acid-residue homologue with snake PLA2, which showed a 90% similarity with other Bothrops moojeni PLA2. BmPLA2 displayed an IC50 of 0.6 M against Caco-2, and demonstrated a selectivity index of 1.85 (compared to MRC-5) and 6.33 (compared to BV-2), supporting its selectivity for cancer cells. In conclusion, we describe a new acidic phospholipase, which showed antitumor activity and is a potential candidate in the development of new biotechnological tools. |
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Thesagro: |
Biotecnologia; Espectrometria; Proteína. |
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Thesaurus Nal: |
Animal proteins; Biotechnology; Bothrops; Cell membranes; Mass spectrometry; Phospholipase A2. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1150743/1/Purification-characterization-evaluation-2022.pdf
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Marc: |
LEADER 02489naa a2200361 a 4500
001 2150743
005 2023-01-06
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.3390/ph15060724$2DOI
100 1 $aFRIHLING, B. E. F.
245 $aPurification, characterization and evaluation of the antitumoral activity of a phospholipase A2 from the snake Bothrops moojeni.$h[electronic resource]
260 $c2022
500 $aNa publicação: Newton Verbisck.
520 $aNature presents a wide range of biomolecules with pharmacological potential, including venomous animal proteins. Among the protein components from snake venoms, phospholipases (PLA2) are of great importance for the development of new anticancer compounds. Thus, we aimed to evaluate the PLA2 anticancer properties from Bothrops moojeni venom. The crude venom was purified through three chromatographic steps, monitored by enzymatic activity and SDS-PAGE (12%). The purified PLA2 denominated BmPLA2 had its molecular mass and N-terminal sequence identified by mass spectrometry and Edman degradation, respectively. BmPLA2 was assayed against human epithelial colorectal adenocarcinoma cells (Caco-2), human rhabdomyosarcoma cells (RD) and mucoepidermoid carcinoma of the lung (NCI-H292), using human fibroblast cells (MRC-5) and microglia cells (BV-2) as a cytotoxicity control. BmPLA2 presented 13,836 Da and a 24 amino acid-residue homologue with snake PLA2, which showed a 90% similarity with other Bothrops moojeni PLA2. BmPLA2 displayed an IC50 of 0.6 M against Caco-2, and demonstrated a selectivity index of 1.85 (compared to MRC-5) and 6.33 (compared to BV-2), supporting its selectivity for cancer cells. In conclusion, we describe a new acidic phospholipase, which showed antitumor activity and is a potential candidate in the development of new biotechnological tools.
650 $aAnimal proteins
650 $aBiotechnology
650 $aBothrops
650 $aCell membranes
650 $aMass spectrometry
650 $aPhospholipase A2
650 $aBiotecnologia
650 $aEspectrometria
650 $aProteína
700 1 $aBOLETI, A. P. de A.
700 1 $aOLIVEIRA, C. F. R. de
700 1 $aSANCHES, S. C.
700 1 $aCARDOSO, P. H. de O.
700 1 $aVERBISCK, N. V.
700 1 $aMACEDO, M. L. R.
700 1 $aSANTA RITA, P. H.
700 1 $aCARVALHO, C. M. E.
700 1 $aMIGLIOLO, L.
773 $tPharmaceuticals$gv. 15, n. 6, article 724, 2022.
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Embrapa Gado de Corte (CNPGC) |
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| 1. |  | ABREU, C. G. de; ROESCH, L. F. W.; ANDREOTE, F. D.; SILVA, S. R.; MORAES, T. S. J. de; ZIED, D. C.; SIQUEIRA, F. G. de; DIAS, E. S.; VARANI, A. M.; PYLRO, V. S. Decoding the chromosome-scale genome of the nutrient-rich Agaricus subrufescens: A Resource for fungal biology and biotechnology. Research in Microbiology, v. 174, n. 8, 104116, 2023.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 3 |
| Biblioteca(s): Embrapa Agroenergia. |
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| 2. | | SILVA, C. de O. G.; CUNHA, R. B.; CONCEIÇÃO, A. A.; ALMEIDA, E. G.; ZIED, D. C.; VIEIRA JUNIOR, W. G. V.; DIAS, E. S.; ISIKHUEMHEN, O. S.; ABDELNUR, P. V.; SIQUEIRA, F. G. de. Outdoor versus indoor cultivation: Effects on the metabolite profile of Agaricus subrufescens strains analyzed by untargeted metabolomics. Food Chemistry, v. 374, 131740, April 2022. 10 p. PDF: il. color.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Agroenergia. |
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| 3. | | PARDO-GIMÉNEZ, A.; FIGUEIRÊDO, V. R. de; ZIED, D. C.; GONZÁLEZ, J. E. P. Sustratos de cobertura y suplementación del compost en cultivo de champiñón. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 47, n. 8, p. 1125-1132, ago. 2012 Título em inglês: Substrate casing and compost supplementation in button mushroom cultivation.| Biblioteca(s): Embrapa Unidades Centrais. |
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| Registros recuperados : 4 | |
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